This study investigated the microbial contamination profiles and antibiotic resistance patterns of bacteria isolated from mobile phones belonging to students, staff, and food handlers at the Federal University of Technology Owerri (FUTO). Thirty mobile phone surfaces (10 from each user group) were sampled using sterile salinemoistened swabs across a standardized 3 cm² area. All sampled devices (100%) showed bacterial contamination, with Staphylococcus aureus being the most prevalent isolate (42.9%), followed by Bacillus cereus (30.6%), Streptococcus spp. (14.2%), Klebsiella spp. (8.2%), and Proteus vulgaris (4.1%). Student devices exhibited the highest diversity of bacterial contaminants. Antimicrobial susceptibility testing revealed gentamicin (17.6%) and pefloxacin (15.1%) as the most effective antibiotics, while erythromycin (0%) and amoxicillin (4.9%) showed the lowest efficacy. These findings demonstrate that mobile phones serve as significant reservoirs for pathogenic bacteria and potential vectors for transmitting multidrug-resistant organisms within academic communities.