In the actual world of contemporary web development, the selection of backend technologies is crucial to the scalability, efficiency, and performance of applications. Node.js and Python are two of the most popular technologies for web application development, each with different strengths based on the use case. This review paper compares Node.js and Python performance in web development based on important parameters like processing speed, scalability, concurrency handling, resource utilization, and ecosystem maturity. By analysing real-world use cases, and community adoption trends, the main aim of this study is to provide guide for the developers and organizations in selecting the appropriate technology for their projects