Musculoskeletal disorders (MSDs) represent the costliest form of work-related disability, accounting for approximately 40% of all expenses associated with occupational injury treatment. This study aimed to assess the prevalence and perceived impacts of MSDs among nurses at Alimosho General Hospital, Lagos State. A cross-sectional descriptiveanalytic quantitative design was employed, utilizing total enumeration of 214 nurses and data collection via the standardized Nordic Musculoskeletal Questionnaire. Descriptive statistical analyses were conducted using Epi Info™ version 7, IBM SPSS Statistics 26.0, and Microsoft Excel 2010. The findings revealed a 12-month prevalence of MSDs in anybody region of 92.5%, with the lower back being the most commonly affected area (64.0%). Both occupational and non-occupational risk factors were significantly associated with MSD development (p < 0.05). However, workplacerelated factors such as prolonged standing (80.6%), repetitive manual tasks (80.7%), and awkward postures (81.4%) emerged as primary contributors. The impact of MSDs on nurses’ work performance was substantial, leading to absenteeism due to pain (71.0%), medical consultation (55.6%), and restriction from normal work activities in the preceding year (56.9%). It is recommended that ongoing educational and awareness programs be implemented to enhance knowledge and prevention of MSDs, particularly focusing on minimizing exposure to occupational risk factors. Additionally, appropriate staffing levels and adequate rest periods are essential to prevent overwork and reduce MSD risk among nursing staff.