Transfusion- associated HBV infection continues to be a major public health problem particularly in developing countries. This study was conducted to determine the prevalence of HBV infection serologic markers among blood donors at Federal Medical Center, Keffi, Nigeria. Blood samples were collected from 200 consenting donors from January to June, 2021. A well-structured questionnaire was used to obtain clinical and socio-demographic information of the patients. The resulting Sera were screened for HBV sero-markers (HBsAg, HBsAb, HBeAg, HBeAb and HBcAb), using the 5- panel HBV combo kit (colloidal gold). Data collected were analysed using statistical package for social science (SPSS), (version 12.0) and pvalue of 0.05). This study reported a high prevalence of HBV infection (8.0%) among prospective blood donors. Hence there is need for proper screening of HBV infection among blood donors to avoid transmission of HBV occult infection.