Food hygiene practices play a vital role in preventing foodborne illnesses, particularly in developing nations such as Nigeria. Market women serve as key stakeholders in ensuring food safety, given their significant role in selling food items to the public. Despite this, limited attention has been given to their perceptions and adherence to proper food hygiene practices. This study, therefore, assessed the perception of market women regarding food hygiene practices among food vendors in Igando, Lagos State. A descriptive research design was adopted, involving 133 market women from the Igando market. The study explored their level of knowledge and how it influences hygiene practices. Findings revealed that although most participants acknowledged the importance of maintaining food hygiene, notable deficiencies existed in both knowledge and practice. For instance, only 45% reported washing their hands before handling food, while 60% did not use gloves during food handling. Furthermore, 70% lacked access to clean water for handwashing, and 80% did not have access to hand sanitizers. The study concludes that there is a pressing need for continuous education and awareness initiatives to enhance market women’s understanding and implementation of food hygiene practices. Strengthening these aspects is crucial for promoting safe food handling and reducing the burden of foodborne diseases in Nigeria.