The demand of bio ethanol as substitute of gasoline had rapidly increased. Today bio ethanol can be produced by dry grind or by wet mill process of food grains (Corn, Millet and Sorghum). Basically, the step involved in the production of bio ethanol includes grinding fermentation and distillation. This project shows bio ethanol production and extraction from the food grains. Also some of the lactic acid bacterial species that were involved in the fermentation process were isolated and identified, as Lactobacillus ferment, Lactobacillus lactics, Lactobacillus planterum, Lactobacillus bulgaricus and Weissella spp. This project work shows that with thehelp of these lactic acid bacteria and others like Saccharomyces cerevisiae, over 6.5% ethanol was obtained by distillation (78°C) 72hours of fermentation. The environmental factors and conditions that after favors the production of ethanol from the substrate sources includes: temperature, pH, anaerobic condition and the sterility of the materials used were put in place to avoid contamination by foreign microorganisms. Standard microbiological procedure was used to determine those lactic acid bacteria that involved in the fermentation ofthe food grains before the distillation was carried out.