This study aimed to evaluate the knowledge and associated factors related to gestational diabetes among pregnant women attending the antenatal clinic at Isolo General Hospital, Lagos State. The specific objectives included assessing the participants' understanding of gestational diabetes, their awareness of its risk factors, and their knowledge of its prevention and management strategies. A simple random sampling method was used to select 167 pregnant women, with 162 respondents ultimately included in the final analysis. Data were gathered using a researcher-designed questionnaire and analyzed using SPSS version 25 and Microsoft Excel 2010, with results presented in frequency and percentage tables. The findings indicated that the participants had a generally good knowledge of gestational diabetes, a moderate awareness of its risk factors, and a good understanding of preventive and management practices. A statistically significant relationship was observed between the participants' level of education and their knowledge of gestational diabetes (p < .05). The study concluded that efforts should be made to maintain and enhance the knowledge and awareness of gestational diabetes and its associated factors among pregnant women.