In Nigeria, the lack of regular cervical cancer screening services and the low uptake of Pap smear screening continue to hinder effective cervical cancer prevention. While visual inspection with acetic acid and cryotherapy have proven to be viable and effective methods for low-resource settings like Nigeria, the adoption of Pap smear screening remains low in many developing and underdeveloped countries. This study aimed to evaluate women's knowledge, attitudes, and practices regarding Pap smear screening. A crosssectional descriptive survey design was employed, conducted in Igando and Ijegun communities, within the Alimosho Local Government Area of Lagos, Nigeria. A convenience sampling technique was used to select participants, and a total of 145 questionnaires were analyzed using the Statistical Package for Social Sciences (SPSS) version 26.0 for descriptive analysis. The findings indicated that the majority of respondents were aged between 32 and 38 years (41.4%), with a mean age of 30.3 ± 7.4 years. Most respondents (37.9%) were married. Regarding education, 24.1% had never attended school, 9.0% had only primary education, 29.0% had completed secondary education, and 37.9% had attained tertiary education. The study revealed that knowledge about Pap smear screening among women was inadequate, which may contribute to the low screening rates and negative attitudes toward the practice. Based on these findings, it is recommended that increased health education campaigns be implemented to raise awareness about Pap smear screening in both rural and urban communities.