Blood donation is commonly regarded as an altruistic act and has been extensively studied. According to a study by Mitra (2010), there is no perfect substitute for blood, as it cannot be artificially manufactured, making blood donation an essential aspect of patient care. This study aimed to assess the knowledge and attitude toward blood donation among residents of Owode Yewa Local Government Area in Ogun State. A prospective descriptive survey design was adopted, utilizing a systematic sampling technique to select 80 residents, regardless of their status. Data was collected using a self-structured questionnaire, with 72 responses correctly completed and retrieved. The data was analyzed using descriptive statistical methods. Findings revealed that the majority of respondents, 62 (86.1%), had good knowledge of blood donation. However, 50 (69.4%) exhibited a poor attitude toward it. Factors such as blood-related illnesses (e.g., leukemia), menstruation, fear of blood, and feelings of weakness after donation were identified as barriers to participation in blood donation. In conclusion, while knowledge of blood donation is high among residents of Owode Yewa, their attitude toward donation remains poor. To address this, the study recommends implementing intensive awareness campaigns to educate and encourage the community, dispel misconceptions, and foster a more positive outlook toward voluntary blood donation.