Zonaria variegata, a species of brown seaweed, has gained attention for its diverse bioactive compounds with potential antimicrobial and antioxidant properties. This study investigates the efficacy of Z. variegata extract in dental applications, particularly its antimicrobial activity against oral pathogens and its potential to inhibit bio film formation. The extract was prepared using methanol extraction and tested against Streptococcus mutans and Shigella Sonnei using the well diffusion method. Additionally, its bio film inhibition and antioxidant properties were evaluated through in vitro assays. The results demonstrated a dose-dependent antimicrobial effect, with significant bio film inhibition and antioxidant activity. These findings suggest that Z. variegata extract could serve as a natural alternative to synthetic antimicrobial agents in dental care. Further studies are required to identify specific bioactive compounds and assess their clinical efficacy.