This study investigated the anti-inflammatory and hepatoprotective effects of aqueousmethanolic extract of soursop (Annona muricata) leaves in formalin-induced inflammation and edema in male rats. Inflammation and oxidative stress are major contributors to liver damage, and soursop leaves have traditional medicinal applications. Thirty male rats were assigned to six groups: normal saline control, formalin-induced control, two treatment groups receiving 100 mg/kg and 300 mg/kg of soursop leaf extract, and two groups treated with standard drugs Diclofenac and Aspirin. Liver function markers, including Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Alkaline Phosphatase (ALP), and total protein, were evaluated. Formalin induction significantly increased AST, ALT, and ALP levels in untreated rats. Treatment with soursop extract at both doses ameliorated ALT and AST elevations, with greater efficacy observed at 300 mg/kg. Diclofenac-treated rats also exhibited significant reductions in these enzymes. Notably, ALP levels were markedly reduced in rats receiving 300 mg/kg extract, indicating restoration of hepatocyte function, while Diclofenac produced a moderate effect. These findings demonstrate that soursop leaf extract effectively mitigates formalin-induced liver enzyme alterations and exhibits significant anti-inflammatory and hepatoprotective activity. The study highlights the therapeutic potential of soursop leaves as a natural intervention for protecting liver function in inflammatory conditions. Keywords: Annona muricata, antiinflammatory, hepatoprotection, formalininduced oedema, liver enzymes.